NUCLEODUR C18 Gravity液相色谱柱


产品简介

NUCLEODUR C18 Gravity液相色谱柱


C18Gravity：PH稳定应用范围宽达1.0—11.0

※     满足您对方法开发
※     极端PH应用的需求
※     和复杂样品的分离


Gravity是在NUCLEODUR纯硅胶基础上，采用 18%高碳载量和多步交联键合技术。特别适合强碱性样品的分析，而且能在PH=1.0—11.0范围内稳定使用,符合美国药典USP L1，常卖货号760101.46C18 Gravity 4.6X250mm 5um



性能：

主要特点：
适用于液相色谱/质谱和高效液相色谱法在PH（PH值1 - 11）
优越的基失活
方法开发的理想
技术特点：
十八烷基改性高密度、多封端
孔径110A；粒径1.8μm，3 m和5 mμ
10，7，12和16μm粒子的制备要
碳含量18%
应用：
整体复杂的分析分离
复合类分离包括药物，如镇痛药、抗炎药、抗抑郁药；除草剂；植物药物；免疫抑制剂



Standard program* of columns packed with NUCLEODUR? C18 Gravity

Column system

粒径:

1.8 μm

3 μm

5 μm

10 μm

分析柱

EC columns (standard system, 2–4.6 mm)

SHAPE * MERGEFORMAT

SHAPE * MERGEFORMAT

+

SHAPE * MERGEFORMAT

+

制备

VarioPrep columns (VP, ≥ 8 mm)

SHAPE * MERGEFORMAT

SHAPE * MERGEFORMAT

+


高效液相色谱分析法


订货信息：

Length ->

30 mm

50 mm

75 mm

100 mm

125 mm

150 mm

250 mm

NUCLEODUR? C18 Gravity, 1.8 μm

2 mm ID

760078.20

760079.20

760071.20

760076.20

760075.20

3 mm ID

760078.30

760079.30

760076.30

4 mm ID

760078.40

760079.40

760076.40

4.6 mm ID

760078.46

760079.46

760076.46

EC guard columns*

4 x 2 mm

761901.20

4 x 3 mm

761901.30

NUCLEODUR? C18 Gravity, 3 μm

2 mm ID

760080.20

760084.20

760081.20

760083.20

760082.20

3 mm ID

760080.30

760084.30

760081.30

760083.30

760082.30

4 mm ID

760080.40

760084.40

760081.40

760083.40

760082.40

4.6 mm ID

760080.46

760086.46

760084.46

760081.46

760083.46

760082.46

EC guard columns*

4 x 2 mm

761902.20

4 x 3 mm

761902.30

NUCLEODUR? C18 Gravity, 5 μm

2 mm ID

760102.20

760104.20

760100.20

760103.20

760101.20

3 mm ID

760102.30

760104.30

760100.30

760103.30

760101.30

4 mm ID

760102.40

760104.40

760100.40

760103.40

760101.40

4.6 mm ID

760102.46

760106.46

760104.46

760100.46

760103.46

760101.46

EC guard columns*

4 x 2 mm

761903.20

4 x 3 mm

761903.30



Guard columns for EC columns

EC columns with ID

Use guard
column holder

2 mm

3 mm

4 mm

4.6 mm

*

Column Protection System

EC guard column

4/2

4/3

4/3

4/3

REF 718966


VarioPrep 柱用在高效液相制备



Length

10 mm ID

21 mm ID

32 mm ID

40 mm ID

NUCLEODUR? C18 Gravity, 5 μm

50 mm

762103.100

762103.210

125 mm

762109.100

762109.210

150 mm

762100.400

250 mm

762113.100

762113.210

762113.320

762113.400

NUCLEODUR? C18 Gravity, 10 μm

250 mm

762250.210

762250.400

VP guard columns with NUCLEODUR? C18 Gravity

8 mm ID

16 mm ID

32 mm ID

10 mm*

762160.80

762160.160

15 mm*

762163.320


* VP guard column

Size

Pack of

for VP column

required holder

REF

VP 10/8

10 x 8 mm ID

2

10 mm ID

8 mm

718251

VP 10/16

10 x 16 mm ID

2

21 mm ID

16 mm

718256

VP 15/32

15 x 32 mm ID

1

32–40 mm ID

32 mm

718253

*对照品舒巴坦钠的

使用NUCLEODUR C18 Gravity和对照柱X按照《药典》方法检测进行

色谱柱：NUCLEODUR C18 Gravity 250×4.6mm 5um  对照柱X 250×4.6mm 5um

流动相：0.005 mol/L 四丁基氢氧化铵（PH=5.0）:乙腈=75:25

温  度：35℃

检测器：UV 230nm

结果:

舒巴坦钠理论塔板数

舒巴坦钠
与杂质分离度

重现性

C18 Gravity

7398

4.13

0.71

对照柱X

6317

4.00

0.15


应用：

1、 万古霉素

Substances:

vancomycin

Column:

EC 250/4.6 NUCLEODUR® C18 Gravity, 5 µm

Phase:

NUCLEODUR® C18 Gravity

REF number:

760101.46

Sample pretreatment:

Sample concentration: 2 mg/mL in eluent A
Stock solution: 4 mL triethylamine + 1996 mL water + H3PO4 (adjusted to pH 3.2)

Conditions:

Eluent A: 920 mL stock solution + 10 mL THF + 70 mL ACN
Eluent B: 700 mL stock solution + 10 mL THF + 290 mL ACN
Gradient: 0 % B for 13 min, 0-100 % B in 9.1 min, 100 % B for 4 min
Flow rate: 1.0 mL/min
Temperature: 25 °C
Injection volume: 20 µL

Detection:

UV, 280 nm

Author(-s):

HPLC department

Source:

MACHEREY-NAGEL, Germany 2010

Keywords:

antibiotics; pharmacopeia; Pharm. Eu.; asymmetry factor

Legend:

1  -  vancomycin

Note:

Vancomycin is determined with an asymmetry factor of 1.12 (Pharm. Eu< 1.60)

Substances:

acetylsalicylic acid; sulindac; piroxicam; suprofen; tolmetin; naproxen; diflunisal; fenoprofen; flurbiprofen; niflumic acid; indomethacin; ibuprofen

Column:

EC 250/4.6 NUCLEODUR® C18 Gravity, 5 µm

Phase:

NUCLEODUR® C18 Gravity

MN catalog number:

760101.46

Conditions:

Mobile phase: acetonitrile / 20 mM KH2PO4, pH 2.5, 45:55 (v/v)
Flowrate: 1.3 ml/min
Temperature: 22 °C
Injection volume: 5.0 µl

Detection:

UV, 230 nm

Author(-s):

HPLC department

Source:

MACHEREY-NAGEL, Germany 2008

Keywords:

antiinflamatoray drugs; drugs; NSAID

Legend:

1   -  acetylsalicylic acid
2   -  sulindac
3   -  piroxicam
4   -  suprofen
5   -  tolmetin
6   -  naproxen
7   -  diflunisal
8   -  fenoprofen
9   -  flurbiprofen
10 -  niflumic acid
11 -  indomethacin
12 -  ibuprofen

Substances:

coumafuryl; warfarin; pindone; coumatetralyl; coumachlor; diphacinone; chlorphacinone; bromadiolone i; difenacoum; bromadiolone ii; flocoumafen i; flocumafen ii; brodifacoum; difethialone; pyranocoumarin

Column:

EC 250/4.6 NUCLEODUR® C18 Gravity, 5 μm
CC 8/4 NUCLEODUR® C18 Gravity, 5 μm (guard column)

Phase:

NUCLEODUR® C18 Gravity

REF number:

760101.46
761125.40

Conditions:

Buffer:
0.68 g KH2PO4 + 0.34 g Na3PO4 x 12 H20 in 1l dest. water, pH 6.3
Eluents:
A: Methanol / Acetonitrile / Buffer (250:100:650)
B: Methanol / Acetonitrile / Buffer (100:650:250)
Flow rate: 1.2 ml/min
Temperature: RT

Detection:

A) DAD UV, 308 nm (285 nm, 260 nm)
B) Fluorescence (stimulation 308 nm / emission 390 nm)

Author(-s):

Freidhof, K.

Source:

LandesuntersuchungsamtRheinland-Pfalz, Koblenz; Germany

Keywords:

anti-coagulant drugs; drugs

Legend:

Peaks:

A)
1) Coumafuryl
2) Warfarin &Pindone
3) Coumatetralyl
4) Coumachlor
5) Diphacinone
6) Chlorphacinone
7) Bromadiolone I
8) Difenacoum
9) Bromadiolone II
10) Flocoumafen I
11) Flocumafen II &Brodifacoum
12) Difethialone
13) Pyranocoumarin

B)
1) Coumafuryl
2) Warfarin
3) Coumatetralyl
4) Coumachlor
5) Bromadiolone I
6) Difenacoum
7) Bromadiolone II
8) Flocoumafen&Brodifacoum
9) Pyranocoumarin



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