qiagen301427-Effectene Transfection Reagent effectene转染试剂
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上海逸研生物科技有限公司是一家销售生物化学试剂及实验耗材的公司主营产品：常规生化试剂，抗体，试剂盒，分离试剂，培养基，血清，细胞及实验室耗材，过滤耗材，食品检测耗材，大气监测耗材，汽车检测耗材等。主要代理有：sigma，Roche，thermo，life，abcam，CST，biovision ，vector,sciencell，whatman，millipore，pall，sartorius等，公司成立以来，秉着诚信经营，服务的理念为广大科研客户及公司带来的服务。
qiagen301427-Effectene Transfection Reagent effectene转染试剂
Effectene Transfection Reagent is an innovative non-l lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids （see figure "Model of Effectene Principle"）, providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods （1, 2）.
The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium （which can contain serum and antibiotics）, and added directly to the cells. The cells are then incubated until harvested and analyzed for gene
Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types （see figures "High Transfection Efficiencies Using Effectene Reagent" and "Transfection of Oligonucleotides Using Effectene Reagent"）. Because transfection can be performed in the presence of serum and requires low amounts of DNA （see figure "Serum and DNA Quantity vs. Transfection Efficiency"）, cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells （see figure "Effectene Reagent with Primary Cells"）. A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.
1. Ausubel, F.M., et al. eds. （1991） Current Protocols in Molecular Biology, Wiley Interscience, New York.
2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. （1989） Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
