探针纯化
探针纯化产品介绍：
Bio–Spin 和微型Bio–Spin 柱可快速纯化DNA 和蛋白样品。填装有特殊规格Bio– Gel P 凝胶的Bio–Spin 和微型Bio–Spin 柱完全能适应水相运载，立刻能使用。这两种 产品可有效地净化DNA、RNA 和蛋白样品，去除盐、核苷酸、染料终止物和小分子等 杂质。优点包括：
预装的预水化凝胶载体，使用方便
快速净化样品，只需在微量离心机上离心4 分钟
有效（98%）去除ddNTPs 和染料终止物
产量提高— DNA（>22 bp）的回收率达95%
上样体积：10 – 100 μl
预装离心柱选择指南
Bio–Spin 6
微型Bio–Spin 6
Bio–Spin 30
微型 Bio–Spin 30
预装载体
级Bio–Gel
P–6 凝胶
级Bio–Gel
P–6 凝胶
级Bio–Gel
P–30 凝胶
级Bio–Gel
P–30 凝胶
平衡缓冲液
10 mM Tris, pH 7.4,
SSC 缓冲液*
10 mM Tris, pH 7.4
或SSC 缓冲液*
10 mM Tris, pH 7.4,
SSC 缓冲液*
10 mM Tris, pH 7.4
或SSC 缓冲液*
应用
对新合成的寡核苷酸
（>20 个碱基）脱盐
—
—
标记反应：从标记DNA 中
去除未结合的标记核苷酸
（>20 个碱基或碱基对）
—
—
酶切片段、PCR 产物、酶切反应和
测序模板的缓冲液置换
—
DNA 测序反应混合物净化**
—
—
RNA 探针净化***
—
—
—
抗体、酶和蛋白溶液的脱盐
—
纯化蛋白（分子量>6,000）
—
—
纯化蛋白（分子量>40,000）
—
—
厂家，
柱床体积
1.1 ml
0.7 ml
1.1 ml
0.7 ml
回收率和滞留率
90% 回收20 个碱基或碱基对：
99% 盐滞留
90% 回收20 个碱基或碱基对；
99% 盐滞留
95% 回收22 个碱基或碱基对；
98% ddNTPs 滞留
95% 回收22 个碱基或碱基对；价格，电话陈生
柱床体积
1.1 ml
0.7 ml
1.1 ml
0.7 ml
回收率和滞留率
90% 回收20 个碱基或碱基对：
99% 盐滞留
90% 回收20 个碱基或碱基对；
99% 盐滞留
95% 回收22 个碱基或碱基对；
98% ddNTPs 滞留
95% 回收22 个碱基或碱基对；
柱床体积
1.1 ml
0.7 ml
1.1 ml
0.7 ml
回收率和滞留率
90% 回收20 个碱基或碱基对：
99% 盐滞留
90% 回收20 个碱基或碱基对；
99% 盐滞留
95% 回收22 个碱基或碱基对；
98% ddNTPs 滞留
95% 回收22 个碱基或碱基对；
98% ddNTPs 滞留
分子量排阻限度，球状蛋白
6,000
6,000
40,000
40,000
样品体积
50–100 μl
10–75 μl
50–100 μl
10–75 μl
离心机类型
水平离心机
微量离心机
水平离心机
微量离心机
高压灭菌
可
可
可
可
* 150 mM NaCl，17.5 mM 柠檬酸钠，pH 7.0
** 溶于Tris 缓冲液中
***溶于不含RNase 的Tris 缓冲液中
目录 #
描述
Bio-Spin Columns With Bio-Gel P-30 in SSC Buffer
732-6006
Bio-Spin 30 Columns, includes 25 columns in SSC buffer, 50 collection tubes
732-6216
Bio-Spin 30 Columns, includes 1,000 columns in SSC buffer, 2,000 collection tubes
Bio-Spin Columns With Bio-Gel P-30 in Tris Buffer
732-6231
Bio-Spin 30 Columns, includes 25 columns in Tris buffer, 50 collection tubes
732-6232
Bio-Spin 30 Columns, includes 100 columns in Tris buffer, 200 collection tubes
732-6233
Bio-Spin 30 Columns, includes 1,000 columns in Tris buffer, 2,000 collection tubes
Bio-Spin Columns With Bio-Gel P-6 in SSC Buffer
732-6002
Bio-Spin 6 Columns, includes 25 columns in SSC buffer, 50 collection tubes
732-6213
Bio-Spin 6 Columns, includes 1,000 columns in SSC buffer, 2,000 collection tubes
Bio-Spin Columns With Bio-Gel P-6 in Tris Buffer
732-6227
Bio-Spin 6 Columns, includes 25 columns in Tris buffer, 50 collection tubes
732-6228
Bio-Spin 6 Columns, includes 100 columns in Tris buffer, 200 collection tubes
732-6229
Bio-Spin 6 Columns, includes 1,000 columns in Tris buffer, 2,000 collection tubes
Micro Bio-Spin Columns With Bio-Gel P-30 in SSC Buffer
732-6202
Micro Bio-Spin 30 Columns, includes 25 columns in SSC buffer, 50 collection tubes
732-6203
Micro Bio-Spin 30 Columns, includes 100 columns in SSC buffer, 200 collection tubes
732-6206
Micro Bio-Spin 30 Columns, includes 1,000 columns in SSC buffer, 2,000 collection tubes
Micro Bio-Spin Columns With Bio-Gel P-30 in Tris Buffer
732-6223
Micro Bio-Spin 30 Columns, includes 25 columns in Tris buffer, 50 collection tubes
732-6224
Micro Bio-Spin 30 Columns, includes 100 columns in Tris buffer, 200 collection tubes
732-6226
Micro Bio-Spin 30 Columns, includes 1,000 columns in Tris buffer, 2,000 collection tubes
Micro Bio-Spin Columns With Bio-Gel P-6 in SSC Buffer
732-6200
Micro Bio-Spin 6 Columns, includes 25 columns in SSC buffer, 50 collection tubes
732-6201
Micro Bio-Spin 6 Columns, includes 100 columns in SSC buffer, 200 collection tubes
732-6205
Micro Bio-Spin 6 Columns, includes 1,000 columns in SSC buffer, 2,000 collection tubes
Micro Bio-Spin Columns With Bio-Gel P-6 in Tris Buffer
732-6221
Micro Bio-Spin 6 Columns, includes 25 columns in Tris buffer, 50 collection tubes
732-6222
Micro Bio-Spin 6 Columns, includes 100 columns in Tris buffer, 200 collection tubes
732-6225
Micro Bio-Spin 6 Columns, includes 1,000 columns in Tris buffer, 2,000 collection tubes
RNase-Free Micro Bio-Spin Columns
732-6250
Micro Bio-Spin 30 Columns, includes 25 columns in Tris buffer, 50 collection tubes, RNase-free
732-6251
Micro Bio-Spin 30 Columns, includes 100 columns in Tris buffer, 200 collection tubes, RNase-free
探针纯化
探针纯化
探针纯化
