Biotinylated Anti Human Oncostatin M peprotech-500-P30Bt

订货号：500-P30Bt

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Biotinylated Anti-Human Oncostatin M

Catalog Number：

500-P30Bt

Source：

Polyclonal Rabbit

Preparation：

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOncostatin M. Anti-Human Oncostatin M specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen：

E.coli derived Recombinant Human Oncostatin M (227 a.a.) (PeproTech catalog #300-10)

Application：

ELISA

Direct

To detect hOncostatin M by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hOncostatin M.

ELISA： Direct

Sandwich

To detect hOncostatin M by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human Oncostatin M (500-P30) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hOncostatin M.

Western Blot

To detect hOncostatin M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Western Transfer (Western Blot)

Research Interests：