胺氧化酶9001-66-5​

发布时间:2021-10-06

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胺氧化酶9001-66-5
英文名称:PAO;Plasma Amine Oxidase
CAS号:9001-66-5
级别:BR
来源:Bovine Plasma
Specific activity:≥17Tabor units/mg
活力定义:1 international unit oxidizes 1μmole of benzylamine per minute at 25℃, pH 7.2
催化反应:
产品描述:Bovine plasma amine oxidase has a molecular weight of 170 kDa and an optimum pH of 6.2 for spermine and 7.2 for spermidine. Amine oxidases are divided into two classes: the pyridoxal and copper containing enzyme to which plasma amine oxidase belongs, and the FAD-containing amino oxidases. Natural substrates include catecholamines, tryptamine derivatives and other physiologically active amines. Plasma amine oxidase is used in research requiring nitrogen group transfers. The molecule is composed of two identical polypeptide chains. There are two pyridoxal phosphates and two atoms of Cu+ per molecule. Bovine plasma amine oxidase is inhibited by copper chelating agents, many carboxyl reagents such as cuprizone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are both non-competitive inhibitors (KI = 30 and 34mM respectively). The assay for determination of amine oxidases employed at Worthington is essentially that of Tabor et al., JBC, 208, 645, (1954) with the reaction temperature reduced to 25℃
性状(以下信息仅供参考):粉末。EC3.1.1.4
用途:本品仅供科研,不得用于其它用途
保存:-20℃

用途:本品仅供科研,不得用于其它用途。(以下用途仅供参考)光度法测定硒的试剂。过氧化酶HRP底物,适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性棕色的末端产物。肉眼或可见光下就可观察。。过氧化酶染色应用:在聚丙烯酰胺凝胶上区分两种分枝杆菌过氧化轻酶(mycobacterial catalase);在聚丙烯酰胺凝胶上血红色蛋白的专一指示剂。
保存:RT,避光

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