lucigen CCIS125 CopyControl™ Induction Solution
产品简介：北京中北林格供应lucigen CCIS125 CopyControl™ Induction Solution，中北林格是lucigen代理，提供lucigen产品货号报价查询。其他包括cell代理，Biosearch technologies代理，epicentre代理，illumina代理，ICLLAB代理，ImmunoReagents代理。
由于生产线调整，自2017年1月1日起，此款产品转至Lucigen™销售， （仅变化，产品外包装变化，本身没有改变，老客户可以继续使用，实验不受影响）
产品名称:CopyControl™ Induction Solution
货号:CCIS125
规格:25 ml
存储温度:-20℃
lucigen CCIS125 CopyControl™ Induction Solution 产品详细说明
Applications
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Preparation of BAC libraries that can be maintained at single copy number and induced to high-copy number as needed.
CopyControl™ BAC Cloning Kits* are complete kits containing all molecular biology reagents needed for constructing high-quality, large-insert Bacterial Artificial Chromosome (BAC) libraries. Each kit has enough reagents to make a complete human genome size library at 10X coverage. The kits feature the CopyControl™ pCC1BAC™ Vector which contains the E. coli F-factor single-copy origin of replication and oriV, a second origin of replication, which can be used to selectively induce to high copy number.
To maintain stability, CopyControl BAC clones are grown at very low copy number during the initial cloning and screening processes. Then, immediately before DNA purification, clones can be induced to 10-20 copies per cell within 2 hours by adding CopyControl Induction Solution to the culture, for higher yields of BAC DNA. The short, 2-hour induction of CopyControl BAC clones to high-copy number does not decrease their stability, d on analysis of Hind III restriction patterns of a large number of induced versus uninduced clones that ranged in size up to ~200 kb. Alternatively, the CopyControl BAC Autoinduction Solution can be used. Autoinduction Solution is added to the culture medium prior to inoculation, and also increases the cell growth leading to higher yield than standard Induction Solution. CopyControl BAC Cloning Kits reduce the time and labor required to construct a BAC library.
Phage T1-resistant TransforMax™ EPI300™-T1R Electrocompetent E. coli cells, or standard TransforMax™ EPI300™ Electrocompetent E. coli cells, which are required for induction of CopyControl BAC clones to 10-20 copies per cell, are available separately.
应用
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BAC文库的制备，可以保持单拷贝数，并根据需要诱导高拷贝数。
CopyControl™BAC克隆试剂盒*是完整的试剂盒，包含构建高质量，大插入细菌人工染色体（BAC）文库所需的所有分子生物学试剂。每个试剂盒都有足够的试剂，可以10倍的覆盖率制作完整的人类基因组大小库。该试剂盒具有CopyControl™pCC1BAC™载体，其含有大肠杆菌 F因子单拷贝复制起点和ori V，第二复制起点，可用于选择性诱导高拷贝数。
为了保持稳定性，CopyControl BAC克隆在初始克隆和筛选过程中以非常低的拷贝数生长。然后，在DNA纯化之前，通过向培养物中添加CopyControl诱导溶液，可以在2小时内将克隆诱导至每个细胞10-20个拷贝，以获得更高的BAC DNA产量。基于对Hin的分析，将CopyControl BAC克隆短时间2小时诱导至高拷贝数不会降低其稳定性d III大量诱导克隆与未诱导克隆的限制性模式，其大小范围可达~200kb。或者，可以使用CopyControl BAC Autoinduction Solution。在接种之前将自诱导溶液添加到培养基中，并且还增加细胞生长，导致比标准诱导溶液更高的产率。CopyControl BAC Cloning Kits减少了构建BAC库所需的时间和人力。
噬菌体T1抗性TransforMax™EPI300™-T1 R电感受态大肠杆菌细胞，或标准TransforMax™EPI300™电感受态大肠杆菌细胞，是将CopyControl BAC克隆诱导至每个细胞10-20个拷贝所必需的，可单独购买。
Benefits
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Safely obtain higher DNA yields while maintaining the stability of single-copy-number clones.
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Constructing BAC libraries is faster and easier.
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CopyControl pCC1BAC cloning vectors are Cloning-Ready: linearized, dephosphorylated, and highly purified.
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Cloning-Ready pCC1BAC vectors give >95% white colonies.
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Estimate the size of the CopyControl BAC clones in as little as 4 hours without culture, Not I digests, or pulse-field gel electrophoresis.
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CopyControl BACs are ideal for use in high-throughput (96-well) purifications when used with the CopyControl BAC Autoinduction solution.
优点
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安全地获得更高的DNA产量，同时保持单拷贝数克隆的稳定性。
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构建BAC库更快更容易。
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CopyControl pCC1BAC克隆载体克隆就绪：线性化，去磷酸化和高度纯化。
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克隆就绪的pCC1BAC载体产生> 95％的白色菌落。
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估计CopyControl BAC克隆的大小只需4小时，无需培养，不进行消化或脉冲场凝胶电泳。
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CopyControl BAC与CopyControl BAC Autoinduction解决方案一起使用时，非常适合用于高通量（96孔）纯化。
lucigen CCIS125 CopyControl™ Induction Solution 图示说明
Figure 1. DNA from induced (+) and uninduced (-) cultures of 4 CopyControl™ BAC clones.An equal number of cells were processed from both induced and uninduced cultures. Samples were resolved on a 0.8% agarose gel and stained with SYBR® Gold (Invitrogen). The induced cultures yielded >15-fold more CopyControl BAC clone DNA. M=BAC-Tracker™ Supercoiled DNA ladder.
图1.来自4个CopyControl TM BAC克隆的诱导（+）和未诱导（ - ）培养物的DNA。从诱导和未诱导的培养物中加工相同数量的细胞。将样品解决在0.8％琼脂糖凝胶上，并用SYBR染色® 金（英杰公司）。诱导的培养物产生> 15倍的CopyControl BAC克隆DNA。M = BAC-Tracker™螺旋DNA梯。
Figure 2. Not I digestion of 2 µl of DNA from induced (+) and uninduced (-) cultures of 4 CopyControl™ BAC clones. After resolving by PFGE and staining with SYBR® Gold (Invitrogen), clone sizes were: BAC1=130 kb, BAC2=125 kb, BAC3=140 kb and BAC4=145 kb. M=Pulsed-Field Gel Marker.
图2. 未从 I CopyControl™BAC克隆的诱导（+）和未诱导（ - ）培养物中消化2μlDNA。 由PFGE和染色用SYBR解决后® 金（英杰公司），克隆大小为：BAC1 = 130 KB，BAC2 = 125 KB，BAC3 = 140 kb和BAC4 = 145 KB。M =脉冲场凝胶标记。
Figure 3 (click to enlarge). The CopyControl™ BAC Cloning Kits significantly reduce the time and labor needed to construct a BAC library compared to standard methods.
图3（点击放大）与标准方法相比，CopyControl™BAC克隆试剂盒显着减少了构建BAC文库所需的时间和人力。
对此产品有兴趣，可联系中北林格获取更多详细信息。
