订货号：500-P168
Anti-Human sTNF Receptor Type II是由上海肯强供应，产品价格优惠，欢迎致电我公司。 手机： QQ：1026257835 邮箱：1026257835@qq.com
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Anti-Human sTNF Receptor Type II
Catalog Number：
500-P168
Source：
Polyclonal Rabbit
Preparation：
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor II. Anti-Human sTNF-receptor II specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor II matrix.
Immunogen：
E.coli derived Recombinant Human sTNF Receptor Type II (PeproTech catalog #310-12)
Application：
ELISA
Sandwich
To detect hsTNF-receptor II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sTNF-receptor II (500-P168Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Western Blot
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Transfer (Western Blot)
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of Human sTNF Receptor-II (0.5 μg/ml) a concentration of 15.0 - 20.0 μg/ml of this antibody is required.
Immunohistochemistry
This antibody stained PC-3 and CaCo-2 cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information is courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Immunofluorescence： General Cell
Research Interests：
| Inflammation | Neurobiology |
| Receptors | TNF Superfamily |
