Anti Human Visfatin peprotech-500-P222

订货号：500-P222

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Anti-Human Visfatin

Catalog Number：

500-P222

Source：

Polyclonal Rabbit

Preparation：

Produced from sera of rabbits immunized with highly pure recombinant Human Visfatin. Anti-Human Visfatin specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix.

Immunogen：

E.coli derived Recombinant Human Visfatin (PeproTech catalog #130-09)

Application：

ELISA

Sandwich

To detect Human Visfatin by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Visfatin (500-P222Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.

ELISA： Sandwich

Western Blot

To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Western Transfer (Western Blot)

Immunohistochemistry

This antibody stained formalin-fixed, paraffin-ded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Antigen Retrieval

Polymer Staining： FFPE Tissue

Country of Origin：

USA

Research Use Only. Not for use in diagnostic or therapeutic procedures.