Biotinylated Anti Human GDF 3 peprotech-500-P235Bt

发布时间:2017-07-11

订货号:500-P235Bt

Biotinylated Anti-Human GDF-3是由上海肯强供应,产品价格优惠,欢迎致电我公司。 手机: QQ:1026257835 邮箱:1026257835@qq.com

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Biotinylated Anti-Human GDF-3

Catalog Number:

500-P235Bt

Source:

Polyclonal Rabbit

Preparation:

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDF-3. Anti-Human GDF-3 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen:

E.coli derived Recombinant Human GDF-3 (PeproTech catalog #120-22)

Application:

ELISA

Direct

To detect hGDF-3 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGDF-3.

ELISA: Direct

Sandwich

To detect hGDF-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human GDF-3 (500-P235) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGDF-3.

Western Blot

To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Western Transfer (Western Blot)

Research Interests:

Diabetes/ Weight Regulation Stem Cells & Differentiation
TGF Superfamily
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