订货号:500-P71Bt
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Biotinylated Anti-Murine SCF
Catalog Number:
500-P71Bt
Source:
Polyclonal Rabbit
Preparation:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF. Anti-Murine SCF specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen:
E.coli derived Recombinant Murine SCF (PeproTech catalog #250-03)
Application:
ELISA
Direct
To detect mSCF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mSCF.
ELISA: Direct
Sandwich
To detect mSCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine SCF (500-P71) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mSCF.
Western Blot
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Transfer (Western Blot)
Research Interests:
Cell Culture | Proliferation |
Stem Cells & Differentiation |