Biotinylated Anti Murine SCF peprotech-500-P71Bt

发布时间:2017-07-11

订货号:500-P71Bt

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Biotinylated Anti-Murine SCF

Catalog Number:

500-P71Bt

Source:

Polyclonal Rabbit

Preparation:

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF. Anti-Murine SCF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen:

E.coli derived Recombinant Murine SCF (PeproTech catalog #250-03)

Application:

ELISA

Direct

To detect mSCF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mSCF.

ELISA: Direct

Sandwich

To detect mSCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine SCF (500-P71) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mSCF.

Western Blot

To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Western Transfer (Western Blot)

Research Interests:

Cell Culture Proliferation
Stem Cells & Differentiation
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