Biotinylated Anti Murine IL 9 peprotech-500-P59Bt

发布时间:2017-07-11

订货号:500-P59Bt

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Biotinylated Anti-Murine IL-9

Catalog Number:

500-P59Bt

Source:

Polyclonal Rabbit

Preparation:

Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-9. Anti-Murine IL-9 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen:

E.coli derived Recombinant Murine IL-9 (PeproTech catalog #219-19)

Application:

ELISA

Direct

To detect Murine IL-9 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-9.

Sandwich

To detect Murine IL-9 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IL-9 (500-P59) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-9.

Western Blot

To detect Murine IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Cross Reactivity:

Mouse

Research Interests:

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