订货号：500-P174G
Anti-Murine EGF是由上海肯强供应，产品价格优惠，欢迎致电我公司。 手机： QQ：1026257835 邮箱：1026257835@qq.com
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Anti-Murine EGF
Catalog Number：
500-P174G
Source：
Polyclonal Goat
Preparation：
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEGF. Anti-Murine EGF specific antibody was purified by affinity chromatography employing immobilized mEGF matrix.
Immunogen：
E.coli derived Recombinant Murine EGF (PeproTech catalog #315-09)
Application：
ELISA
Sandwich
To detect mEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine EGF (500-P174GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF.
ProtocolItem/ELISA： Sandwich
Western Blot
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ProtocolItem/Western Transfer (Western Blot)
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of Murine EGF (0.1 ng/ml), a concentration of 3.0-5.0 ng/ml of this antibody is required.
Research Interests：
| Angiogenesis/ Cardiovascular | Cancer |
| Cell Culture | Inflammation |
| Neurobiology | Proliferation |
| Stem Cells & Differentiation | Wound Healing |
