
订货号: 500-P122G
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Anti-Rat IFN-γ
Catalog Number:
500-P122G
Source:
Polyclonal Goat
Preparation:
Produced from sera of goats pre-immunized with highly pure recombinant Rat IFN-γ. Anti-Rat IFN-γ specific antibody was purified by affinity chromatography employing immobilized Rat IFN-γ matrix.
Immunogen:
E.coli derived Recombinant Rat IFN-γ (PeproTech catalog #400-20)
Application:
ELISA
Sandwich
To detect Rat IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IFN-γ (500-P122GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-γ.
ELISA: Sandwich
Western Blot
To detect Rat IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Transfer (Western Blot)
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IFN-γ (0.3 ng/ml), a concentration of 60.0-65.0 ng/ml of this antibody is required.
Cross Reactivity:
Rat
Research Interests:
| AIDS/ HIV | Angiogenesis/ Cardiovascular |
| Cancer | Diabetes/ Weight Regulation |
| Immune System | Neurobiology |
| Stem Cells & Differentiation | Allergy |
| Transplantation |
