Anti- SOX11抗体说明书

FROM:ABCAM

Catalog Number : ab50194

Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Background:
All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance which may affect binding and interactions of other tranion factors. SOX1 SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse (Collignon et al. 1996). These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. SOX11 appears to be important in the developing nervous system (SOX11 is detected from 8.5 dpc through to 13.5 dpc with a considerable increase in by 13.5 dpc). SOX11 is apparent in the somiteslimbsfacial mesenchymekidneylung of the fetal system.

pecificity :   

-    Anti- SOX11 is a rabbit  polyclonal antibody unconjugated                 

specific for SOX11 of   ratmouse

–         use for western blottingelisaimmunoprecipitation and immunohistochemistry

–         Protein G affinity chromatography purification purity :>95%

–         Isotype:  IgG

Application :  

–        WB=1:100-500  

–        ELISA=1:500-1000  

–        IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 ，not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least six weeks at 2-4 ℃

 

Important Note:This product as supplied is intended for research use only not for use in human therapeutic or diagnostic applications.