Anti-BDNF抗体说明书
Catalog Number :RS-0248R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background:
Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF) originally discovered in the 1950s as a soluble peptide promoting the survival of and neurite outgrowth from sympathetic ganglia. More recently three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF) neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3 while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family.
Specificity : Anti-BDNF is a rabbit monoclonal antibody
specific for BDNF of humanrabbit mouse rat，cow
– use for western blottingelisaimmunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification purity :>95%
– Isotype: IgG
– mol wt: 12.5 kDa
Application :
– WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 lmmunohistochem1：100-500
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only not for use in human therapeutic or diagnostic applications.
