Rabbit Anti-SP1(transcription factor Sp1)

      Rabbit Anti-SP1(tranion factor Sp1)

Catalog Number :RS-0975R

Quantity size : 0.2ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )

Background :

Profound changes in patterns of gene can result from relatively small changes in the concentrations of sequence specific tranion factors. Synergistic interaction between factors bound to different sites within a tranional control region is supported by the work of Courey et al. (1989). Sp1 is a sequence specific tranion factor that recognizes GGGGCGGGGC and closely related sequences which are often referred to as GC boxes. Sp1 binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. SP1 can interact with G/C rich motifs from serotonin receptor promoter. Kadonaga et al. (1987) cloned the human Sp1 cDNA and showed that it has contiguous zinc finger motifs and requires zinc for sequence specific binding to DNA. Altername：Sp1 tranion factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Tranion factor Sp1.

 

Specificity :

· anti- SP1 is a rabbit monoclonal antibody unconjugated

·· specific for SP1 of humanrat mouse and chicken

· use for elisa immunoprecipitation and immunohistochemistry

· affinity chromatography purification purity :>95%

· Isotype: IgG

 

Application:

 · Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

 

Storage:

 Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note:

This product as supplied is intended for research use only not for use in human therapeutic or diagnostic applications.