Human Interleukin 6 KIT英文实验原理

Principle of the assay
Human Interleukin 6 kit assay Human Interleukin 6(IL-6) level in the sample，use Purified Human Interleukin 6(IL-6)antibody to coat microtiter plate wells make solid-phase antibody then add Interleukin 6(IL-6) to wells Combined antibody which With HRP labeled goat anti- Human become antibody - antigen - enzyme-antibody complex after washing Completely Add TMB substrate solutionTMB substrate becomes blue color At HRP enzyme-catalyzed reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of  Human Interleukin 6(IL-6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.