SMAD3抗体说明书

                  Anti-SMAD3抗体说明书

Catalog Number : Rs-3484R

Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation differentiation and development. The Smad family is divided SMAD3抗体SMAD3抗体说明书into three subclasses: receptor regulated Smads activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1 5 and 8); the common partner (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus allowing its binding to DNA and tranion factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 tranional activity and TGF beta signaling.

pecificity :   

- Anti- smad3 is a rabbit polyclonal antibody unconjugated                 

specific fo smad3 of  Human Mouse Rat Chicken Cow

• use for western blottingelisaimmunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification purity :>95%
• Isotype:  IgG
• mol wt:  47kDa

Application :  

• WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody the antibody is stable for at least six weeks at 2-4 ℃

 

Important Note: This product as supplied is intended for research use only not for use in  human therapeutic or diagnostic applications.SMAD3抗体SMAD3抗体说明书