Anti-Smad7抗体antibody，免疫组化试剂盒说明书
（Mothers against decapentaplegic homolog 7）
Catalog Number : Rs-0566R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Antagonist of signaling by TGF-beta （transforming growth factor） type 1 receptor superfamily members; has been shown to inhibit TGF-beta （Transforming growth factor） and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1 which promotes its dephosphorylation. [SUBCELLULAR LOCATION] Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm. Belongs to the dwarfin/SMAD family. Smad7 is transforms the growth factor （TGF-β） the signal passage to suppress the member Smad7 may intervene the MAPK signal passage causes ERK and JNK phosphorylation active balanced being out of balance causes to press the multiplication to affect strongly in the growth inhibitory action thus is helpful in the cell develops to the malignant direction
Specificity : · anti- Smad7 is a rabbit polyclonal antibody unconjugated
· specific for Smad7 of human mouse rat bov pig
· epitope mapping near the N-terminus of Smad7 of human mouse rat;
bov; pig
· use for western blotting elisa immunoprecipitation
and immunohistochemistry
· Protein G affinity chromatography purification purity :>95%
· Isotype: IgG
· mol wt: 46kDa
· Immunohistochemistry 1:100-500
·ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only not for use in
human therapeutic or diagnostic applications.
Anti-Smad7抗体antibody，免疫组化试剂盒说明书www.hbzhan.com/st44025
