Anti-ACACA抗体说明书，abcam一抗
From:ABCAMCatalog Number :RS- 11912R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background:cetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA the rate-limiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACC alpha (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids and ACC∫ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC alpha is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA which serve as the enzyme’s short-term regulatory mechanism. The gene encoding ACC alpha maps to human chromosome 17 and encodes a form of ACC which is the major ACC in lipogenic tissues. The catalytic core of ACC∫ is homologous to that of the ACCå except for an additional peptide of about 150 amino acids at the N-terminus.
Function : Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein biotin carboxylase and carboxyltransferase.
Subunit : Monomer homodimer and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.Subcellular Location : Cytoplasm.Tissue Specificity : Expressed in brain placental skeletal muscle renal pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.Post-translational modifications : Phosphorylation on Ser-1263 is required for interaction with BRCA1.DISEASE : Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage persistent myopathy and poor growth.Similarity : Contains 1 ATP-grasp domain.Contains 1 biotin carboxylation domain.Contains 1 biotinyl-binding domain.Contains 1 carboxyltransferase domain.
.Specificity : Anti- ACACA is a rabbit polyclonal antibody
specific for ACACA of Human Mouse Rat Dog Pig Cow Horse Rabbit Sheep
– use for western blottingelisaimmunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification purity :>95%
– Isotype: IgG -mol wt: 266kDa Anti-ACACA抗体说明书，abcam一抗
Application :
– WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
– KLH conjugated synthetic peptide derived from human ACACA
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only not for use in human therapeutic or diagnostic applications.
Anti-ACACA抗体说明书，abcam一抗
