Anti-His tag抗体，单抗，多抗说明书
From:ABCAM
Catalog Number :RS- 0287R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background:The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
.Specificity : Anti- His tag is a rabbit polyclonal antibody
specific for His tagof fusionproteinHHHHHHtagged，mouse
– use for western blottingelisaimmunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification purity :>95%
– Isotype: IgG
Application : Anti-His tag抗体，单抗，多抗说明书
– WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
– Synthetic peptide: HHHHHH conjugated to KLH
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only not for use in human therapeutic or diagnostic applications.
Anti-His tag抗体，单抗，多抗说明书
