Rabbit Anti-NF-M
Cat. Number: RS-0710R
Quantity size: 0.1ml (dilute with pH 7.4 0.01 M PBS or diluent of antibody)
Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons and are composed predominantly of three major proteins called neurofilament light (NF-L) neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases such as Lou Gehrig&s disease or Alzheimer&s disease.
Specificity: · anti- NF-M is a Rabbit monoclonal antibody unconjugated
· specific for NF-M of human rat and chicken
· epitope mapping near the C terminus of NF-M of human rat
and chicken
· human rat and chicken cross reacted
· use for western blotting elisa immunoprecipitation
and immunohistochemistry
· Protein A affinity chromatography purification purity :>95%
· Isotype: IgG · mol wt: 48kDa
Rabbit Anti-NF-M
Application: · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only not for use in human therapeutic or diagnostic applications.
Rabbit Anti-NF-M
